Precision Mosquito Control with
Sterile Insect Technique

How Can We Suppress the Population of Invasive Mosquitoes?

Without using chemicals, worrying about resistance development, or GMO (genetically modified organism).

Request a Field Trial Survey for Mosquito Control Professionals Survey for Community Members
Illustration of the yellow fever mosquito, Aedes aegypti.

What is SIT and how it is done at Sterile Insect Farms?

Sterile Insect Technique (SIT) is a form of biological pest control for insects, often mosquitoes, but also fruit flies, moths, and other pests. Sterile Insect Farms (SIF) uses Rad Source’s X-ray irradiation system to sterilize adult insects, then organizations release them in large numbers into the wild. It’s an environmentally friendly method that reduces insecticide use while maintaining ecological balance.1

Sterile Insect Technique (SIT) a method already cutting wild mosquito populations by nearly 82% in a Greater Los Angeles County Vector Control District pilot program.

How SIT works, five steps: mass rearing, sorting, sterilization, mass release, population suppression.

Inside the Farm

Mosquito larvae rearing in a shallow tray of water.
Larval pans
A gloved technician preparing a blood feed for the colony.
Blood feeding
Adult mosquitoes resting on the mesh wall of a rearing cage.
Adults feeding on sugar water
COMMUNITY OUTREACH

What a Mosquito Release Program Means for a Neighborhood

Most residents have never heard of the Sterile Insect Technique before it comes to their area, and that unfamiliarity is common.

SIT programs are already running in neighborhoods across California and Florida, with strong community support once people understand how it works. Here are the five questions every district hears first.

Are you releasing more mosquitoes near my house?

Male mosquitoes only. Males feed on plant nectar the same way honeybees do. They have no interest in you, and they cannot bite even if they wanted to. Only females bite, and only because they need protein to develop eggs.2

Is anything radioactive?

No. X-ray irradiation directs high-energy photons at the insect, the same physics behind a dental X-ray. Nothing radioactive is used, and nothing radioactive is left behind in the mosquito. Once the machine is off, the X-rays are gone.3

Is this a GMO?

No, DNA is not inserted, edited, or engineered. The mosquito we release is the same species already living in your area. The only difference is that it cannot produce offspring that hatch.1

Will this hurt bees, butterflies, or birds?

SIT is species-specific. Sterile male Aedes aegypti only mate with female Aedes aegypti. Pollinators, fish, birds, and bats are unaffected, and the released males are safe if something eats them.1

Does anything stay behind in the environment?

The released males live a normal short life and die. No bacterium spreads, no gene persists, and no residue is left in the soil or water.

Female Aedes aegypti Target Humans

Most female mosquitoes will feed on whatever host is nearby, such as birds, deer, or household pets. Aedes aegypti is different: it has evolved over thousands of years alongside humans and preferentially seeks out human blood, even when other hosts are available.7

Unlike many mosquito species, Aedes aegypti is a daytime feeder, so bed nets offer little protection. It tends to bite low, around the ankles and feet, where its presence often goes unnoticed. Because it typically takes several small blood meals to produce a single batch of eggs, one infected mosquito can potentially transmit a virus like dengue to multiple people in a single day.8, 9

The species also breeds close to where people live. Its eggs and larvae can develop in astonishingly small amounts of standing water, a bottle cap, a clogged gutter, a plant saucer, a tarp fold. As a result, the mosquito responsible for a bite is often one that developed within a hundred meters of that location.10

CDC map showing the estimated potential range of Aedes aegypti in the United States, 2017.
These maps represent CDC’s best estimate of the potential range of Ae. aegypti and Ae. albopictus in the United States. Maps do not represent risk for spread of disease. Map courtesy of the Centers for Disease Control and Prevention.
SIT compared to Wolbachia (IIT)

SIT vs Wolbachia

If you’ve followed recent developments in mosquito control, you’ve likely encountered the term Wolbachia. It’s worth understanding, because the underlying mechanism, and what it introduces into the environment, differs fundamentally from irradiation-based approaches like SIT.

Rad Source method

Sterile insect technique (X-ray)

If a female is accidentally released: still sterile, cannot establish in the wild

Introduces to the environment: nothing that outlives the insect

Track record: 70+ years, used on many pest species

Advantage: non-GMO, no chemical approach

Stays effective: an accidental release poses no risk to the technique’s long-term reliability

Alternative method

Wolbachia  (IIT)

If a female is accidentally released: can establish Wolbachia in the wild population

Introduces to the environment: a bacterium, requires ongoing monitoring

Track record: 17+ years as a mosquito control tool

Disadvantage: once established, the Wolbachia infection spreads on its own through the local population and can’t be recalled

Risk of accidental release: an escaped infected female can spread Wolbachia into the wild population, undermining future control

01

What is Wolbachia ?

Wolbachia is a bacterium found naturally in roughly half of all insect species, including many bees and butterflies.11 It does not normally live in Aedes aegypti.12 When researchers introduce it, it creates a form of sterility called cytoplasmic incompatibility. A male carrying Wolbachia mates with a wild female, and her eggs do not hatch.13

02

What happens if a female slips through in the Wolbachia  program?

Sorting males from females is mechanical, and at the volumes these programs run, nothing is perfect. Wolbachia passes only from mother to offspring, so a carrier female that mates with a wild male produces a full brood that inherits the bacterium.13 Given enough generations, Wolbachia spreads until nearly every mosquito in the area carries it, permanently changing the local population and ending the suppression effect.14

03

Why we use X-rays

A precise X-ray dose sterilizes every insect in the batch, male and female. A female that slips through can still fly and still mate, but she cannot produce viable eggs. No offspring means no inherited bacterium and no lineage to start a population shift.15

Sign up for a Field Trial

Sterile Insect Farms ships male Ae. aegypti that have already been X-ray sterilized, sorted, and packaged for field release. Your team opens the shipment on-site and releases them directly, with no additional handling required.

A program in Lee County, Florida applied this same approach and reduced the wild Ae. aegypti population by up to 79%, using X-ray sterilization alone, without Wolbachia.4

Do you want to run a field trial, or replenish your existing egg stock? Fill out the form below and our team at Sterile Insect Farms will follow up to discuss what a program could look like for your district.

Sterile Insect Farms Mass Rearing Capabilities

  • Male Aedes aegypti Adults Ready for release

Support SIT by Taking a Quick Survey

If you support Sterile Insect Technique, show it. Fill out our quick survey, it takes less than 5 minutes, and get a free digital copy of our poster, “Breaking the Cycle, Not the Ecosystem,” as a thank-you you can proudly display!

Homeowners and business owners can use any email. If you’re with a mosquito control district or professional organization, please use your work email so we can route your response correctly.

Survey for Community Members Survey for Mosquito Control Professionals
Poster titled Breaking the Cycle, not the Ecosystem, showing illustrations of the yellow fever mosquito, codling moth, Mediterranean fruit fly, screwworm fly, and screwworm larvae.

SOURCES

  1. International Atomic Energy Agency / FAO. Sterile Insect Technique: Principles and Practice in Area-Wide Integrated Pest Management, 2nd ed. (2021). iaea.org/publications/14775
  2. Centers for Disease Control and Prevention. “Mosquito Life Cycle: Aedes aegypti.” cdc.gov/mosquitoes
  3. U.S. Food and Drug Administration. “Radiation Emitting Products: Medical X-ray Imaging.” fda.gov/radiation-emitting-products
  4. Morreale R, et al. “Sterile insect technique for suppression of Aedes aegypti in Lee County, Florida.” PLOS Neglected Tropical Diseases 19(7): e0013256 (2025). 79% reduction in wild adults, Phase 1. Equipment: Rad Source 2400 V at 42 Gy. journals.plos.org/plosntds
  5. Birhanie SK, et al. “Reduction in Aedes aegypti Population After a Year-Long Application of Targeted Sterile Insect Releases in the West Valley Region of Southern California.” Insects 16(1): 81 (2025). Peer reviewed and open access. Equipment: Rad Source RS 1800·Q at 55 Gy. mdpi.com/2075-4450/16/1/81
  6. Multiple field trials of irradiated Ae. albopictus, including the 2025 Switzerland small-scale trial. pmc.ncbi.nlm.nih.gov/PMC12372186
  7. Rose NH, et al. “Climate and Urbanization Drive Mosquito Preference for Humans.” Current Biology 30(18): 3570-3579 (2020). cell.com/current-biology
  8. Centers for Disease Control and Prevention. “Dengue Transmission” (daytime biting). cdc.gov/dengue/transmission
  9. Scott TW, et al. “Blood-feeding patterns of Aedes aegypti: Multiple feeding per gonotrophic cycle.” Journal of Medical Entomology 30(1): 94-99 (1993). pubmed.ncbi.nlm.nih.gov/8433350
  10. Moore TC, Brown HE. “Estimating Aedes aegypti (Diptera: Culicidae) Flight Distance: Meta-Data Analysis.” Journal of Medical Entomology (2022). Mean flight distance 106 meters across 38 studies. doi.org/10.1093/jme/tjac070
  11. Weinert LA, Araujo-Jnr EV, Ahmed MZ, Welch JJ. “The incidence of bacterial endosymbionts in terrestrial arthropods.” Proceedings of the Royal Society B 282: 20150249 (2015). Estimates 52% of arthropod species carry Wolbachia. royalsocietypublishing.org/rspb.2015.0249
  12. Hilgenboecker K, et al. “How many species are infected with Wolbachia?” FEMS Microbiology Letters 281(2): 215-220 (2008). academic.oup.com/femsle
  13. Werren JH, Baldo L, Clark ME. “Wolbachia: master manipulators of invertebrate biology.” Nature Reviews Microbiology 6: 741-751 (2008). Cytoplasmic incompatibility and maternal-only inheritance. nature.com/articles/nrmicro1969
  14. Turelli M, Hoffmann AA. Spread dynamics of Wolbachia through wild populations. See also the World Mosquito Program’s own documentation of permanent establishment as the intended outcome of their replacement strategy. worldmosquitoprogram.org
  15. Zheng X, et al. “Incompatible and sterile insect techniques combined eliminate mosquitoes.” Nature 572: 56-61 (2019). Establishes low-dose irradiation as the safeguard that sterilizes residual females in a Wolbachia release program. nature.com/articles/s41586-019-1407-9